Dolphin sperm cytoplasmic ribosomes

The use of cryopreserved dolphin spermatozoa facilitates the exchange of genetic material between aquatic parks and makes spermatozoa accessible to laboratories for studies to further our understanding of marine mammal reproduction. Heterologous IVF, a replacement for homologous IVF, could provide a means to test the sperm fertility potential; to study gamete physiology and early embryo development; and to avoid the use of valuable dolphin oocytes, which are difficult to obtain. Here, we present protocols that have been successfully used to collect and cryopreserve dolphin spermatozoa. The collection of semen is performed by manual stimulation on trained dolphins. Cryopreservation is accomplished using a TRIS egg-yolk based extender with glycerol. In addition, we present a protocol that describes heterologous IVF using dolphin spermatozoa and bovine oocytes and that verifies the hybrid nature of the resulting embryo using PCR.
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24.10.2018
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Dolphin Sperm Cytoplasmic Ribosomes mature bottom men

To browse Academia. Skip to main content. You're using an out-of-date version of Internet Explorer. Log In Sign Up. Heterologous murine and bovine IVF using bottlenose dolphin Tursiops truncatus spermatozoa Theriogenology, Eva Martinez-Nevado. Heterologous murine and bovine IVF using bottlenose dolphin Tursiops truncatus spermatozoa.
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